Advancing osteoporosis medicine by observing bone microstructure and remodelling using a four-dimensional nanoscope
Advancing osteoporosis medicine by observing bone microstructure and remodelling using a four-dimensional nanoscope
(Third Party Funds Single)
Overall project:
Project leader: ,
Project members: , , , , ,
Start date: April 1, 2019
End date: March 31, 2025
Acronym: 4-D nanoSCOPE
Funding source: European Research Council (ERC)
URL: https://cordis.europa.eu/project/id/810316
Abstract
Due to Europe's ageing society, there has been a dramatic increase in the occurrence of osteoporosis (OP) and related diseases. Sufferers have an impaired quality of life, and there is a considerable cost to society associated with the consequent loss of productivity and injuries. The current understanding of this disease needs to be revolutionized, but study has been hampered by a lack of means to properly characterize bone structure, remodeling dynamics and vascular activity. This project, 4D nanoSCOPE, will develop tools and techniques to permit time-resolved imaging and characterization of bone in three spatial dimensions (both in vitro and in vivo), thereby permitting monitoring of bone remodeling and revolutionizing the understanding of bone morphology and its function.
To advance the field, in vivo high-resolution studies of living bone are essential, but existing techniques are not capable of this. By combining state-of-the art image processing software with innovative 'precision learning' software methods to compensate for artefacts (due e.g. to the subject breathing or twitching), and innovative X-ray microscope hardware which together will greatly speed up image acquisition (aim is a factor of 100), the project will enable in vivo X-ray microscopy studies of small animals (mice) for the first time. The time series of three-dimensional X-ray images will be complemented by correlative microscopy and spectroscopy techniques (with new software) to thoroughly characterize (serial) bone sections ex vivo.
The resulting three-dimensional datasets combining structure, chemical composition, transport velocities and local strength will be used by the PIs and international collaborators to study the dynamics of bone microstructure. This will be the first time that this has been possible in living creatures, enabling an assessment of the effects on bone of age, hormones, inflammation and treatment.
Publications
Calibration by differentiation – Self-supervised calibration for X-ray microscopy using a differentiable cone-beam reconstruction operator
In: Journal of Microscopy (2022)
ISSN: 0022-2720
DOI: 10.1111/jmi.13125
BibTeX: Download
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Learned Cone-Beam CT Reconstruction Using Neural Ordinary Differential Equations
7th International Conference on Image Formation in X-Ray Computed Tomography (Baltimore, June 12, 2022 - June 16, 2022)
In: Proceedings Volume 12304, 7th International Conference on Image Formation in X-Ray Computed Tomography; 1230409 (2022) 2022
DOI: 10.1117/12.2646442
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Optimizing CT Scan Geometries With and Without Gradients
17th International Meeting on Fully Three-Dimensional Image Reconstruction in Radiology and Nuclear Medicine (Fully3D) (Stony Brook University, New York, July 16, 2023 - July 21, 2023)
Open Access: https://arxiv.org/abs/2302.06251
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Geometric Constraints Enable Self-Supervised Sinogram Inpainting in Sparse-View Tomography
17th International Meeting on Fully Three-Dimensional Image Reconstruction in Radiology and Nuclear Medicine (Fully3D) (Stony Brook University, NY, USA, July 16, 2023 - July 21, 2023)
DOI: 10.48550/arXiv.2302.06436
URL: https://arxiv.org/pdf/2302.06436.pdf
BibTeX: Download
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Ultra low‐parameter denoising: Trainable bilateral filter layers in computed tomography
In: Medical Physics 49 (2022), p. 5107-5120
ISSN: 0094-2405
DOI: 10.1002/mp.15718
BibTeX: Download
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